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Biology Teaching and Learning Centre · ANU College of Science and Medicine
This course provides an introduction to the principles and practice of recombinant DNA technology. It has a focus on how biological processes can be exploited and manipulated for practical purposes, rather than how they operate in nature. The course also introduces some of the main problem solving skills used routinely in molecular biology. A wide range of methods and applications will be discussed including: polymerase chain reaction (PCR), DNA cloning, DNA sequencing, gene libraries, blotting techniques, expression of recombinant proteins, gene mapping, transgenic animals, and gene therapy.
4 x workshop tests (25%; LO 1, 2, 4)
Practical lab exercises (journal and report 25%; LO 1, 3, 4)
Final exam (50%; LO 1, 2, 3)
Two lectures per week, up to eight laboratory sessions of up to four hours each and eight one-hour workshops.
Brown, T.A. (2010) Gene Cloning and Analysis: an introduction (6th ed.) Wiley-Blackwell